Gene dosage analysis in Azotobacter vinelandii.
نویسندگان
چکیده
منابع مشابه
Gene dosage analysis in Azotobacter vinelandii.
For more than a decade, Azotobacter vinelandii has been considered a polyploid bacterium on the basis of physical studies of chromosome size and DNA content per cell. However, as described in the present work, many genetic operations can be performed in A. vinelandii without the constraints expected in a polyploid bacterium: (i) reversion of transposon-induced mutations is usually associated wi...
متن کاملGenes in Azotobacter vinelandii
Azotobacter vinelandii contains a heterodimeric, membrane-bound [NiFelhydrogenase capable of catalyzing the reversible oxidation of H2. The 13 and at subunits of the enzyme are encoded by the structural genes hoxK and hoxG, respectively, which appear to form part of an operon that contains at least one further potential gene (open reading frame 3 1ORF3]). In this study, determination of the nuc...
متن کاملPlasmids of Azotobacter vinelandii.
Four laboratory strains and two isolates of Azotobacter vinelandii were found to contain plasmids. Twenty-five laboratory strains which could fix nitrogen did not have free, covalently closed circular plasmid DNA. The plasmids varied in size from 9 to 52 megadaltons, and each strain yielded only one plasmid. No discernible differences in ability to fix nitrogen were found between plasmid-bearin...
متن کاملUltrastructure of Azotobacter vinelandii.
Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and mi...
متن کاملSuccinic dehydrogenase of Azotobacter vinelandii.
During the investigation of the Krebs cycle in Azotobacter agile, strain 4.4 (Repaske and Wilson, 1953), it was found that whole cells normally required 20 minutes before adaptation to succinate, but succinoxidase activity could be demonstrated in cell-free extracts made from unadapted cells. If extracts prepared in a 10 kc Raytheon sonic oscillator were centrifuged at 145,000 G for 30 minutes,...
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ژورنال
عنوان ژورنال: Genetics
سال: 1992
ISSN: 1943-2631
DOI: 10.1093/genetics/132.4.869